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244k custom gene expression g4502a-07® microarrays  (Agilent technologies)


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    Agilent technologies 244k custom gene expression g4502a-07® microarrays
    244k Custom Gene Expression G4502a 07® Microarrays, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/244k+g4502a+microarray/pmc05896072-112-15-16
    Average 90 stars, based on 1 article reviews
    244k custom gene expression g4502a-07® microarrays - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Gene Expression:

    Article Title: The "go or grow" potential of gliomas is linked to the neuropeptide processing enzyme carboxypeptidase E and mediated by metabolic stress.
    Article Snippet: Glioblastoma (GBM), the most common malignant brain tumor, is among the most lethal neoplasms, with a median survival of approximately 1 year.. Prognosis is poor since GBMs possess a strong migratory and highly invasive potential, making complete surgical resection impossible.. Reduced expression of carboxypeptidase E (CPE), a neuropeptide-processing enzyme, in a cell deathresistant glioma cell line and lower CPE expression levels in the cohort of GBM samples of The Cancer Genome Atlas compared to normal brain control specimens prompted us to analyze the function of CPE as a putative tumor suppressor gene.

    Article Title: Endothelial Wnt/β-catenin signaling inhibits glioma angiogenesis and normalizes tumor blood vessels by inducing PDGF-B expression
    Article Snippet: .. Gene expression signatures were analyzed in 424 primary GBMs and 11 normal brain samples by assessing the TCGA data portal that used the 244K G4502A microarray (Agilent Technologies) to determine mRNA profiles for Wnt1, Wnt3a, Wnt5a, Wnt7a, Wnt7b, Dkk1, and Dkk2 to correlate the expression to normal brain ( https://tcga-data.nci.nih.gov/tcga/tcgaHome2.jsp , accessed 2012 March 30; ). .. The raw data for the selected genes were downloaded and analyzed in Prism (GraphPad Software) to generate scatter blots.

    Article Title: MIF Receptor CD 74 is Restricted to Microglia/Macrophages, Associated with a M 1‐Polarized Immune Milieu and Prolonged Patient Survival in Gliomas
    Article Snippet: Immunodetection was performed by HRP enzyme‐coupled secondary antibodies which oxidized luminol (Santa Cruz Biotechnology, Heidelberg, Germany) resulting in a chemoluminescent reaction visible on processed X‐ray films (Super RX, Fujifilm Europe GmbH, Düsseldorf, Germany). .. The cancer genome atlas ( TCGA ), the R epository of M olecular B rain N eoplasia D ata ( REMBRANDT ) and R 2: microarray analysis and visualization platform analyses To determine gene expression of the MIF‐CD74 system in gliomas, we additionally analyzed data from the TCGA using the Agilent 244K G4502A microarray to determine mRNA profiles ( http://tcga.cancer.gov/ ; accessed November 2011). ..

    Microarray:

    Article Title: The "go or grow" potential of gliomas is linked to the neuropeptide processing enzyme carboxypeptidase E and mediated by metabolic stress.
    Article Snippet: Glioblastoma (GBM), the most common malignant brain tumor, is among the most lethal neoplasms, with a median survival of approximately 1 year.. Prognosis is poor since GBMs possess a strong migratory and highly invasive potential, making complete surgical resection impossible.. Reduced expression of carboxypeptidase E (CPE), a neuropeptide-processing enzyme, in a cell deathresistant glioma cell line and lower CPE expression levels in the cohort of GBM samples of The Cancer Genome Atlas compared to normal brain control specimens prompted us to analyze the function of CPE as a putative tumor suppressor gene.

    Article Title: Endothelial Wnt/β-catenin signaling inhibits glioma angiogenesis and normalizes tumor blood vessels by inducing PDGF-B expression
    Article Snippet: .. Gene expression signatures were analyzed in 424 primary GBMs and 11 normal brain samples by assessing the TCGA data portal that used the 244K G4502A microarray (Agilent Technologies) to determine mRNA profiles for Wnt1, Wnt3a, Wnt5a, Wnt7a, Wnt7b, Dkk1, and Dkk2 to correlate the expression to normal brain ( https://tcga-data.nci.nih.gov/tcga/tcgaHome2.jsp , accessed 2012 March 30; ). .. The raw data for the selected genes were downloaded and analyzed in Prism (GraphPad Software) to generate scatter blots.

    Article Title: MIF Receptor CD 74 is Restricted to Microglia/Macrophages, Associated with a M 1‐Polarized Immune Milieu and Prolonged Patient Survival in Gliomas
    Article Snippet: Immunodetection was performed by HRP enzyme‐coupled secondary antibodies which oxidized luminol (Santa Cruz Biotechnology, Heidelberg, Germany) resulting in a chemoluminescent reaction visible on processed X‐ray films (Super RX, Fujifilm Europe GmbH, Düsseldorf, Germany). .. The cancer genome atlas ( TCGA ), the R epository of M olecular B rain N eoplasia D ata ( REMBRANDT ) and R 2: microarray analysis and visualization platform analyses To determine gene expression of the MIF‐CD74 system in gliomas, we additionally analyzed data from the TCGA using the Agilent 244K G4502A microarray to determine mRNA profiles ( http://tcga.cancer.gov/ ; accessed November 2011). ..

    Expressing:

    Article Title: Endothelial Wnt/β-catenin signaling inhibits glioma angiogenesis and normalizes tumor blood vessels by inducing PDGF-B expression
    Article Snippet: .. Gene expression signatures were analyzed in 424 primary GBMs and 11 normal brain samples by assessing the TCGA data portal that used the 244K G4502A microarray (Agilent Technologies) to determine mRNA profiles for Wnt1, Wnt3a, Wnt5a, Wnt7a, Wnt7b, Dkk1, and Dkk2 to correlate the expression to normal brain ( https://tcga-data.nci.nih.gov/tcga/tcgaHome2.jsp , accessed 2012 March 30; ). .. The raw data for the selected genes were downloaded and analyzed in Prism (GraphPad Software) to generate scatter blots.



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    CD74 mRNA and protein levels correlate with the content of glioma‐associated microglia/macrophages (GAMs). Hierarchical cluster analyses (A) of gene expression signatures of selected key factors of immune cells, glioblastoma cells as well as glioblastoma micromilieu of 424 primary glioblastomas and 11 normal brain samples deriving from the cancer genome atlas (TCGA) data portal using the Agilent <t>244K</t> <t>G4502A</t> microarray to determine mRNA profiles. Hierarchical clustering of this data was performed using the Ward's minimum variance method. Dark red color indicates a perfect positive correlation (+1) gradually decreasing to a perfect negative correlation (−1) indicated in blue. Correlation analyses of the same data subset comparing CD74 with either (B) Iba1 (AIF1), (C) CD68 or (D) CD11b (ITGAM) are depicted. CD74 and Iba1 immunoblotting (E) of GAMs, glioblastoma cells (PC) and whole glioblastoma tissue (T) of three different patients with primary glioblastoma World Health Organization (WHO) grade IV showing a positive correlation of CD74 and Iba1 protein expression. Actin served as positive control.
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    CD74 mRNA and protein levels correlate with the content of glioma‐associated microglia/macrophages (GAMs). Hierarchical cluster analyses (A) of gene expression signatures of selected key factors of immune cells, glioblastoma cells as well as glioblastoma micromilieu of 424 primary glioblastomas and 11 normal brain samples deriving from the cancer genome atlas (TCGA) data portal using the Agilent <t>244K</t> <t>G4502A</t> microarray to determine mRNA profiles. Hierarchical clustering of this data was performed using the Ward's minimum variance method. Dark red color indicates a perfect positive correlation (+1) gradually decreasing to a perfect negative correlation (−1) indicated in blue. Correlation analyses of the same data subset comparing CD74 with either (B) Iba1 (AIF1), (C) CD68 or (D) CD11b (ITGAM) are depicted. CD74 and Iba1 immunoblotting (E) of GAMs, glioblastoma cells (PC) and whole glioblastoma tissue (T) of three different patients with primary glioblastoma World Health Organization (WHO) grade IV showing a positive correlation of CD74 and Iba1 protein expression. Actin served as positive control.
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    Image Search Results


    CD74 mRNA and protein levels correlate with the content of glioma‐associated microglia/macrophages (GAMs). Hierarchical cluster analyses (A) of gene expression signatures of selected key factors of immune cells, glioblastoma cells as well as glioblastoma micromilieu of 424 primary glioblastomas and 11 normal brain samples deriving from the cancer genome atlas (TCGA) data portal using the Agilent 244K G4502A microarray to determine mRNA profiles. Hierarchical clustering of this data was performed using the Ward's minimum variance method. Dark red color indicates a perfect positive correlation (+1) gradually decreasing to a perfect negative correlation (−1) indicated in blue. Correlation analyses of the same data subset comparing CD74 with either (B) Iba1 (AIF1), (C) CD68 or (D) CD11b (ITGAM) are depicted. CD74 and Iba1 immunoblotting (E) of GAMs, glioblastoma cells (PC) and whole glioblastoma tissue (T) of three different patients with primary glioblastoma World Health Organization (WHO) grade IV showing a positive correlation of CD74 and Iba1 protein expression. Actin served as positive control.

    Journal: Brain Pathology

    Article Title: MIF Receptor CD 74 is Restricted to Microglia/Macrophages, Associated with a M 1‐Polarized Immune Milieu and Prolonged Patient Survival in Gliomas

    doi: 10.1111/bpa.12194

    Figure Lengend Snippet: CD74 mRNA and protein levels correlate with the content of glioma‐associated microglia/macrophages (GAMs). Hierarchical cluster analyses (A) of gene expression signatures of selected key factors of immune cells, glioblastoma cells as well as glioblastoma micromilieu of 424 primary glioblastomas and 11 normal brain samples deriving from the cancer genome atlas (TCGA) data portal using the Agilent 244K G4502A microarray to determine mRNA profiles. Hierarchical clustering of this data was performed using the Ward's minimum variance method. Dark red color indicates a perfect positive correlation (+1) gradually decreasing to a perfect negative correlation (−1) indicated in blue. Correlation analyses of the same data subset comparing CD74 with either (B) Iba1 (AIF1), (C) CD68 or (D) CD11b (ITGAM) are depicted. CD74 and Iba1 immunoblotting (E) of GAMs, glioblastoma cells (PC) and whole glioblastoma tissue (T) of three different patients with primary glioblastoma World Health Organization (WHO) grade IV showing a positive correlation of CD74 and Iba1 protein expression. Actin served as positive control.

    Article Snippet: The cancer genome atlas ( TCGA ), the R epository of M olecular B rain N eoplasia D ata ( REMBRANDT ) and R 2: microarray analysis and visualization platform analyses To determine gene expression of the MIF‐CD74 system in gliomas, we additionally analyzed data from the TCGA using the Agilent 244K G4502A microarray to determine mRNA profiles ( http://tcga.cancer.gov/ ; accessed November 2011).

    Techniques: Expressing, Microarray, Western Blot, Positive Control